A girl who Shinkuro lived with when he was young and also his sparring partner. Her soft demeanor (albeit with yandere tendencies, triggered by other girls approaching Shinkuro) hides the fact that she is a strong fighter, whom Shinkuro acknowledges to be much stronger than himself, despite her being older by just one year. Yuno is upset that Shinkuro has not realized he was her first love. Even now, she still has feelings for Shinkuro, always finding excuses to spend time with him, such as cooking meals for him. However, her advances tend to be very threatening (most likely because Shinkuro always has another girl with him) and scare him into just letting her hear what she wants. Yuno resents the decision Shinkuro made in deciding to move out her household to live on his own and fears he will change into a hooligan. She has made it very clear that she wants him back in the Hozuki household. Yuno has a younger sister named Chizuru, who is five, who also has a crush on Shinkuro. Even though the Hozuki family are no longer assassins, their grandfather expects Yuno and Chizuru to carry on the Hozuki family martial arts.
In the anime, Yuno is much more assertive in her advances towards Shinkuro and does not appear to have the yandere tendencies.